Taq Polymerase Dealers in Surat

• Cat. No: ULSC003
• Preparation: 1000 U
• Result Type: Qualitative
• Usage: Hospital & Clinical
• Storage: Cool & Dry Place
• Life: Long shelf life

Features Taq DNA polymerase is a thermostable DNA polymerase isolated and purified from E.coli strain that carries a plasmid with cloned Taq DNA polymerase. It is lacks 3’-5’ proofreading activity and there is a 5’-3’exonuclease activity in the same polypeptide as the DNA polymerase. Taq is designed for use in DNA amplification, primer extension reaction and DNA sequencing.   Advantages & Features : Highest purity : > 98% confirmed by SDS-PAGE. Highest quality : high activity, specificity, thermo stability and performance in PCR. Highly efficient : reactivation buffer improved. Thermostable : half-life at 94º C is 40 minutes. Adds extra nucleotides : preferentially adenine, without template at 3´ends leaving 3´overhangs PCR fragments. Incorporates modified nucleotides : biotinylated, fluorescently labelled, etc. Molecular Weight : 94 kDa. Convenient : available in different concentrations, sizes and solutions. Complete solution : includes MgCl2.

• Type: Pure
• Form: Liquid
• Color: Transparent
• Use in: Laboratory
• Grade: Superior
• Storage: Cool & Dry Place

Taq DNA Polymerase is a thermo stable DNA polymerase that exhibits a 5’→3’ polymerase activity and a 5’→3’exonuclease activity, with no 3’→5’exonuclease activity. Taq DNA Polymerase is purified from an Escherichia coli (E.coli) strain over expressing the gene of Thermus aquaticus DNA polymerase. Taq DNA polymerase is heat-stable and will synthesize DNA at elevated temperatures from single-stranded templates in the presence of a primer. Taq Polymerase is recommended for use in routine PCR reactions. We supplied Taq Polymerase with appropriate buffers. Usually 1-1.5 u of Taq DNA Polymerase is used in 50 µl of reaction mix. Higher Taq DNA Polymerase concentrations may cause synthesis of nonspecific products. However, if inhibitors are present in the reaction mix (e.g., if the template DNA used is not highly purified), higher amounts of Taq DNA polymerase (2-3 u) may be necessary to obtain a better yield of amplification products. No endonuclease, exonuclease, or bacterial DNA were detected in this kit.